";s:4:"text";s:2034:" The nitrocellulose membrane protects proteins that are exposed to acidic–basic conditions or organic solvents. Composition, properties, and manufacture of nitrocellulose Cellulose is a naturally occurring polymer obtained from wood pulp or the short fibres (linters) that adhere to cotton seeds.
Third, nitrocellulose membranes can be cast that High Retention of Small Proteins: The 0.2 μm pore size of the Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through’. It is classified as an explosive when nitrogen content exceeds 12.6 percent. Second, chemistries that make the membrane wettable with aqueous solution do not significantly diminish protein adsorption. First, nitrocellulose adsorbs protein at a high level.
It is effective for detecting low amounts of proteins and also provides an opportunity to determine sequencing manipulations of high molecular weight proteins ( Young and Hongbao, 2006; Shively, 2000 ). PVDF vs Nitrocellulose Membranes The first protein electroblotting experiments were performed using Millipore ® nitrocellulose membrane i, as the Western blotting method was derived from an earlier technique for identification … Thus, this membrane is perhaps the most versatile platform for protein immobilization. Nitrocellulose Membrane Support Nitrocellulose has been used in traditional protein blotting methods, high throughput arrays, mass spectrometry proteomics and immunodiagnostics. Nitrocellulose is produced by nitric acid reacting with cellulose.